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Reverse Complement
Reverse Complement converts a DNA sequence into its reverse, complement, or reverse-complement counterpart. You may want to work with the reverse-complement of a sequence if it contains an ORF on the reverse strand.
NEB Tm Calculator
Use the NEB Tm Calculator to estimate an appropriate annealing temperature when using NEB PCR products.
NEBIO Calculator
This tool will calculate the mass of insert required at several molar insert:vector ratios in the range needed for typical ligation reactions.